5-methylcytosine (5mC) is an epigenetic modification to DNA which modulates transcription. 5mC might be sequentially oxidized to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Collectively, these marks are known as the oxidized derivatives of 5mC (i.e., oxi-mCs). Their formation is catalyzed by the ten-eleven translocation methylcytosine dioxygenases (TETs 1, 2 and three).
Numerous methods have been developed for the detection of oxi-mCs. The next chapter describes an immunochemical protocol for the simultaneous detection of 5hmC and 5caC in embryonic zebrafish tissue sections. The embryos are fastened, permeabilized and embedded in paraffin blocks. The blocks are lower into sections which can be mounted onto slides. Depurination of the DNA is carried out to permit immunodetection of the oxi-mCs. The 5hmC is detected with the assistance of a mouse anti-5hmC monoclonal major antibody and a goat anti-mouse Alexa Fluor 633-conjugated secondary antibody. The weak 5caC sign requires enzymatic amplification. Its detection entails a rabbit anti-5caC polyclonal major antibody and a goat anti-rabbit secondary antibody that’s conjugated to horseradish peroxidase (HRP).
HRP amplifies the 5caC sign by catalyzing the deposition of huge portions of fluorescein-labeled tyramide. Sections immunostained for 5hmC and 5caC are analyzed by fluorescent gentle or confocal laser scanning microscopy. This immunochemical technique permits for extremely delicate detection of 5hmC and 5caC in zebrafish tissues. Ameloblastoma is a domestically invasive odontogenic neoplasm that has a excessive recurrence price. The invasion of adjoining tissue is supported by angiogenesis stimulated by Tumour-Related Macrophages (TAMs). TAMs are macrophages modified within the milieu of the tumour microenvironment and have very weak or no means to current antigens. Thus, there’s collaboration between the tumour and the tumour microenvironment to take care of tumour enlargement.
Enhancement of tradition of Legionella longbeachae from respiratory samples utilizing immunomagnetic separation and antimicrobial decontamination
BackgroundLegionella longbeachae is the most common Legionella species recognized in sufferers with neighborhood acquired pneumonia in New Zealand. Isolation of the organism on tradition is the gold customary for the analysis of Legionnaires illness, however has poor sensitivity (40%) in contrast with qPCR. Now we have developed a selective decontamination course of utilizing glycine, vancomycin, polymyxin and cycloheximide (GVPC) with immunomagnetic separation (IMS) for culturing L. longbeachae.MethodsA polyclonal antibody particular for L. longbeachae was produced from New Zealand white rabbits and matched to tosylactivated magnetic beads.
Saved L. longbeachae qPCR constructive respiratory samples have been retrieved from -80°C storage for testing. One portion of check samples have been combined with GVPC and the antibody beads complicated, separated, washed and cultured on MWY agar. One other portion was uncovered to HCl:KCl acidic buffer (pH 2.2) earlier than incubation on MWY agar. qPCR used probes particular for the ITS (inner transcribed spacer) area of the L. longbeachae genome. Anaphylaxis secondary to thymoglobulin (anti-thymocyte globulin) is a uncommon situation that may be life threatening.
Thymoglobulin is a rabbit-derived T-cell depleting polyclonal immunoglobulin. It’s generally used for induction immunosuppression and/or for remedy of acute rejection in renal transplantation. We report a case of a residing kidney transplant recipient who developed intraoperative anaphylactic shock secondary to thymoglobulin. The affected person had a historical past of pet rabbit publicity. This case report highlights the significance of immediate identification and administration of intraoperative anaphylaxis, which is vital to a profitable final result. Induction immunosuppression choice primarily based on affected person traits is essential. Communication between the anesthesia group and surgeons performed a key position in stopping the donor surgical procedure.
Environment friendly immunodiagnosis of Citrus yellow mosaic virus utilizing polyclonal antibodies with an expressed recombinant virion-associated protein.
Citrus yellow mosaic virus (CYMV) is a member of genus Badnavirus of the household Caulimoviridae. It’s the causal agent of citrus yellow mosaic illness in citrus and causes discount in yield. Because the virus is vegetative propagated by grafting, growth of high-throughput analysis strategies primarily based on serological methods is a prerequisite for manufacturing of wholesome virus-free planting materials.
The present examine describes the event of polyclonal antibodies raised in rabbits in opposition to purified recombinant virion-associated protein (rVAP) encoded by ORF-II of CYMV. The specificity of developed antiserum was evaluated in immunosorbent electron microscopy (ISEM), antigen-coated plate-enzyme linked immunosorbent assay (ACP-ELISA) and immunocapture PCR (IC-PCR). The antiserum particularly reacted as much as a dilution of 1:2000 in ACP-ELISA for detection of CYMV-infected crops. The antiserum was validated by screening CYMV-infected crops maintained within the glass home by means of ACP-ELISA.
Rabbit anti Chicken IgG (rhodamine) |
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| 43C-CB0324 | Fitzgerald | 1.5 mg | EUR 315 |
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Description: Rabbit anti Chicken IgG secondary antibody (Rhodamine) |
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Rabbit anti Dog IgG (rhodamine) |
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| 43C-CB0410 | Fitzgerald | 1.5 mg | EUR 318 |
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Description: Rabbit anti Dog IgG secondary antibody (Rhodamine) |
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Rabbit anti Dog IgG (rhodamine) |
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| 43C-CB0417 | Fitzgerald | 2 mg | EUR 302 |
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Description: Rabbit anti Dog IgG secondary antibody (Rhodamine) |
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Rabbit anti Goat IgG (rhodamine) |
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| 43C-CB0524 | Fitzgerald | 2 mg | EUR 291 |
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Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
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Rabbit anti Goat IgG (rhodamine) |
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| 43C-CB0531 | Fitzgerald | 2 mg | EUR 291 |
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Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
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Rabbit anti Goat IgG (rhodamine) |
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| 43C-CB0538 | Fitzgerald | 1 mg | EUR 284 |
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Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
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Rabbit anti Human IgG (rhodamine) |
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| 43C-CB0945 | Fitzgerald | 1 mg | EUR 348 |
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Description: Rabbit anti Human IgG secondary antibody (Rhodamine) |
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Rabbit anti Human IgG (rhodamine) |
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| 43C-CB0952 | Fitzgerald | 2 mg | EUR 278 |
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Description: Rabbit anti Human IgG secondary antibody (Rhodamine) |
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Rabbit anti Human IgG (rhodamine) |
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| 43C-CB0966 | Fitzgerald | 2 mg | EUR 291 |
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Description: Rabbit anti Human IgG secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (rhodamine) |
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| 43C-CB1138 | Fitzgerald | 2 mg | EUR 280 |
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Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (rhodamine) |
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| 43C-CB1145 | Fitzgerald | 2 mg | EUR 330 |
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Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (rhodamine) |
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| 43C-CB1152 | Fitzgerald | 2 mg | EUR 289 |
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Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (rhodamine) |
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| 43C-CB1159 | Fitzgerald | 2 mg | EUR 388 |
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Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
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Rabbit anti Rat IgG (rhodamine) |
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| 43C-CB1245 | Fitzgerald | 1.5 mg | EUR 304 |
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Description: Rabbit anti Rat IgG secondary antibody (Rhodamine) |
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Rabbit anti Rat IgG (rhodamine) |
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| 43C-CB1252 | Fitzgerald | 2 mg | EUR 291 |
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Description: Rabbit anti Rat IgG secondary antibody (Rhodamine) |
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Rabbit anti Rat IgM (rhodamine) |
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| 43C-CB1266 | Fitzgerald | 1.5 mg | EUR 303 |
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Description: Rabbit anti Rat IgM secondary antibody (Rhodamine) |
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Rabbit anti Sheep IgG (rhodamine) |
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| 43C-CB1324 | Fitzgerald | 1.5 mg | EUR 280 |
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Description: Rabbit anti Sheep IgG secondary antibody (Rhodamine) |
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Rabbit anti Sheep IgG (rhodamine) |
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| 43C-CB1331 | Fitzgerald | 500 µL | EUR 414 |
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Description: Rabbit anti Sheep IgG secondary antibody (Rhodamine) |
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Rabbit anti Sheep IgG (rhodamine) |
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| 43C-CB1338 | Fitzgerald | 1.5 mg | EUR 301 |
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Description: Rabbit anti Sheep IgG secondary antibody (Rhodamine) |
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Rabbit anti Sheep IgG (rhodamine) |
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| 43C-CB1345 | Fitzgerald | 500 µL | EUR 399 |
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Description: Rabbit anti Sheep IgG secondary antibody (Rhodamine) |
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Rabbit anti Mouse IgM (rhodamine) |
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| 43C-CB5108 | Fitzgerald | 1.5 mg | EUR 298 |
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Description: Rabbit anti Mouse IgM secondary antibody (Rhodamine) |
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Rabbit anti Mouse IgG1 (rhodamine) |
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| 43C-CB5122 | Fitzgerald | 1 mg | EUR 369 |
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Description: Rabbit anti Mouse IgG1 secondary antibody (Rhodamine) |
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Rabbit anti Mouse IgG2b (rhodamine) |
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| 43C-CB5136 | Fitzgerald | 1 mg | EUR 369 |
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Description: Rabbit anti Mouse IgG2b secondary antibody (Rhodamine) |
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Rabbit anti Mouse IgG3 (rhodamine) |
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| 43C-CB5138 | Fitzgerald | 1 mg | EUR 369 |
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Description: Rabbit anti Mouse IgG3 secondary antibody (Rhodamine) |
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Rabbit anti Mouse IgA (rhodamine) |
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| 43C-CB5250 | Fitzgerald | 1 mg | EUR 280 |
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Description: Rabbit anti Mouse IgA secondary antibody (Rhodamine) |
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Rabbit anti Human IgM (rhodamine) |
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| 43C-CB9102 | Fitzgerald | 1.5 mg | EUR 302 |
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Description: Rabbit anti Human IgM secondary antibody (Rhodamine) |
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Rabbit anti Human IgA (rhodamine) |
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| 43C-CB9116 | Fitzgerald | 1 mg | EUR 301 |
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Description: Rabbit anti Human IgA secondary antibody (Rhodamine) |
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Rabbit anti Mouse IgG2a (rhodamine) |
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| 43R-IR011rd | Fitzgerald | 1 mg | EUR 369 |
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Description: Rabbit anti Mouse IgG2a secondary antibody (Rhodamine) |
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Rabbit anti Mouse Kappa Chain (rhodamine) |
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| 43C-CB5157 | Fitzgerald | 1 mg | EUR 397 |
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Description: Rabbit anti Mouse Kappa Chain secondary antibody (Rhodamine) |
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Rabbit anti Mouse Lambda Chain (rhodamine) |
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| 43C-CB5164 | Fitzgerald | 1 mg | EUR 365 |
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Description: Rabbit anti Mouse Lambda Chain secondary antibody (Rhodamine) |
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Rabbit anti Goat IgG Fc (rhodamine) |
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| 43R-1083 | Fitzgerald | 1 mg | EUR 263 |
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Description: Rabbit anti Goat IgG Fc fragment secondary antibody (rhodamine) |
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Rhodamine B |
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| HY-Y0016 | MedChemExpress | 1g | EUR 160 |
Rhodamine B |
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| AT031 | Unibiotest | 1mg | EUR 1368 |
Rhodamine 800 |
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| HY-101876 | MedChemExpress | 50mg | EUR 173 |
Rhodamine 6G |
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| HY-D0309 | MedChemExpress | 10mM/1mL | EUR 126 |
Rhodamine 123 |
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| HY-D0816 | MedChemExpress | 25mg | EUR 173 |
Rhodamine 110 |
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| HY-D0817 | MedChemExpress | 100mg | EUR 147 |
Rhodamine Phalloidin |
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| HY-K0903 | MedChemExpress | 300T | EUR 843 |
Rhodamine B |
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| AG031 | Unibiotest | 1 mg | EUR 523 |
Rhodamine 123 |
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| abx082227-5mg | Abbexa | 5 mg | EUR 217 |
Rhodamine B |
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| abx082254-5g | Abbexa | 5 g | EUR 175 |
Rhodamine B |
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| RB0464 | Bio Basic | 100g | EUR 69.14 |
Ubiquitin-Rhodamine |
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| 6411-50 | Biovision | EUR 544 | |
Rhodamine B |
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| TBP03900 | ChemNorm | 6X100mg | EUR 257 |
Rhodamine antibody |
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| 10C-CR2050M1 | Fitzgerald | 500 ug | EUR 446 |
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Description: Mouse monoclonal Rhodamine antibody |
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Rabbit anti Bovine IgG (H + L) (rhodamine) |
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| 43C-CB0103 | Fitzgerald | 2 mg | EUR 249 |
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Description: Rabbit anti Bovine IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Cat IgG (H + L) (rhodamine) |
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| 43C-CB0203 | Fitzgerald | 2 mg | EUR 249 |
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Description: Rabbit anti Cat IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Chicken IgG (H + L) (rhodamine) |
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| 43C-CB0303 | Fitzgerald | 1.5 mg | EUR 271 |
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Description: Rabbit anti Chicken IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Dog IgG (H + L) (rhodamine) |
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| 43C-CB0403 | Fitzgerald | 2 mg | EUR 251 |
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Description: Rabbit anti Dog IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Goat IgG (H + L) (rhodamine) |
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| 43C-CB0503 | Fitzgerald | 2 mg | EUR 249 |
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Description: Rabbit anti Goat IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Goat IgG (H + L) (rhodamine) |
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| 43C-CB0510 | Fitzgerald | 1 mg | EUR 291 |
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Description: Rabbit anti Goat IgG (H + L) secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43C-CB1103 | Fitzgerald | 2 mg | EUR 227 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43C-CB1110 | Fitzgerald | 2 mg | EUR 275 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43C-CB1117 | Fitzgerald | 1 mg | EUR 375 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (Rhodamine) |
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Donkey anti Rabbit IgG (H + L) (rhodamine) |
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| 43C-CB1124 | Fitzgerald | 2 mg | EUR 225 |
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Description: Donkey anti Rabbit IgG (H + L) secondary antibody (Rhodamine) |
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Donkey anti Rabbit IgG (H + L) (rhodamine) |
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| 43C-CB1131 | Fitzgerald | 1 mg | EUR 380 |
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Description: Donkey anti Rabbit IgG (H + L) secondary antibody (Rhodamine) |
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Sheep anti Rabbit IgG (H + L) (rhodamine) |
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| 43C-CB1166 | Fitzgerald | 2 mg | EUR 234 |
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Description: Sheep anti Rabbit IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Rat IgG (H + L) (rhodamine) |
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| 43C-CB1203 | Fitzgerald | 2 mg | EUR 245 |
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Description: Rabbit anti Rat IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Rat IgG (H + L) (rhodamine) |
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| 43C-CB1210 | Fitzgerald | 1 mg | EUR 309 |
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Description: Rabbit anti Rat IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Sheep IgG (H + L) (rhodamine) |
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| 43C-CB1310 | Fitzgerald | 2 mg | EUR 259 |
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Description: Rabbit anti Sheep IgG (H + L) secondary antibody (Rhodamine) |
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Rabbit anti Sheep IgG (H + L) (rhodamine) |
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| 43C-CB1317 | Fitzgerald | 1 mg | EUR 310 |
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Description: Rabbit anti Sheep IgG (H + L) secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (Fab'2) (rhodamine) |
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| 43C-CJ0409 | Fitzgerald | 1 mg | EUR 329 |
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Description: Goat anti Rabbit IgG secondary antibody (Fab'2) (Rhodamine) |
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Goat anti Rabbit IgG (Fab'2) (rhodamine) |
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| 43C-CJ0414 | Fitzgerald | 500 µL | EUR 271 |
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Description: Goat anti Rabbit IgG secondary antibody (Fab'2) (Rhodamine) |
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Rabbit anti Goat IgG (H + L) (rhodamine) |
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| 43R-1059 | Fitzgerald | 1 mg | EUR 155 |
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Description: Rabbit anti Goat IgG (H + L) secondary antibody (rhodamine) |
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Rabbit anti Goat IgG (H + L) (rhodamine) |
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| 43R-1065 | Fitzgerald | 1 mg | EUR 171 |
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Description: Rabbit anti Goat IgG (H + L) secondary antibody (rhodamine) |
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Rabbit anti Hamster IgG (H + L) (rhodamine) |
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| 43R-1109 | Fitzgerald | 1 mg | EUR 149 |
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Description: Rabbit anti Hamster IgG (H + L) secondary antibody (rhodamine) |
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Rabbit anti Human IgG (H + L) (rhodamine) |
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| 43R-1273 | Fitzgerald | 1 mg | EUR 149 |
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Description: Rabbit anti Human IgG (H + L) secondary antibody (rhodamine) |
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Rabbit anti Human IgM (mu chain) (rhodamine) |
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| 43R-1279 | Fitzgerald | 1 mg | EUR 326 |
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Description: Rabbit anti Human IgM (mu chain) secondary antibody (rhodamine) |
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Rabbit anti Mouse IgG (H + L) (rhodamine) |
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| 43R-1419 | Fitzgerald | 1 mg | EUR 149 |
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Description: Rabbit anti Mouse IgG (H + L) secondary antibody (rhodamine) |
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Rabbit anti Mouse IgG (H + L) (rhodamine) |
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| 43R-1425 | Fitzgerald | 1 mg | EUR 171 |
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Description: Rabbit anti Mouse IgG (H + L) secondary antibody (rhodamine) |
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Chicken anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1431 | Fitzgerald | 1 mg | EUR 263 |
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Description: Chicken anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Chicken anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1437 | Fitzgerald | 500 ug | EUR 186 |
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Description: Chicken anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Donkey anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1439 | Fitzgerald | 1 mg | EUR 147 |
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Description: Donkey anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Donkey anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1445 | Fitzgerald | 1 mg | EUR 182 |
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Description: Donkey anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Donkey anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1450 | Fitzgerald | 1 mg | EUR 205 |
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Description: Donkey anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1455 | Fitzgerald | 1 mg | EUR 137 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1461 | Fitzgerald | 1 mg | EUR 138 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1467 | Fitzgerald | 1 mg | EUR 138 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1473 | Fitzgerald | 1 mg | EUR 138 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1478 | Fitzgerald | 1 mg | EUR 205 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1484 | Fitzgerald | 500 ug | EUR 171 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1490 | Fitzgerald | 500 ug | EUR 186 |
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Description: Goat anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Sheep anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1502 | Fitzgerald | 1 mg | EUR 153 |
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Description: Sheep anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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Rabbit anti Rat IgG (H + L) (rhodamine) |
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| 43R-1555 | Fitzgerald | 1 mg | EUR 171 |
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Description: Rabbit anti Rat IgG (H + L) secondary antibody (rhodamine) |
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Goat anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-1616 | Fitzgerald | 1 mg | EUR 219 |
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Description: Goat anti Rabbit IgG (H + L) (rhodamine) secondary antibody |
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Donkey anti Rabbit IgG (H + L) (rhodamine) |
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| 43R-RD002RD | Fitzgerald | 500 ug | EUR 802 |
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Description: Donkey anti Rabbit IgG (H + L) secondary antibody (rhodamine) |
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HCV Nucleocapsid [Rhodamine] |
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| DAG1390 | Creative Diagnostics | 100 µg | EUR 810 |
Rhodamine B [HRP] |
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| DAGA-060H | Creative Diagnostics | 1mg | EUR 1209 |
Rhodamine B [BSA] |
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| DAGA-072B | Creative Diagnostics | 1mg | EUR 780 |
Rhodamine B [KLH] |
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| DAGA-078K | Creative Diagnostics | 1mg | EUR 1079 |
Rhodamine phalloidin 300u |
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| 00027 | Biotium | 300 | EUR 345 |
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Description: Minimum order quantity: 1 unit of 300 |
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Rhodamine 110 phalloidin |
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| 00032 | Biotium | 300ST | EUR 345 |
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Description: Minimum order quantity: 1 unit of 300ST |
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Rhodamine-DHPE: (5mg) |
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| 60026 | Biotium | 5MG | EUR 226 |
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Description: Minimum order quantity: 1 unit of 5MG |
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Octadecyl rhodamine B |
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| 60033 | Biotium | 10MG | EUR 281 |
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Description: Minimum order quantity: 1 unit of 10MG |
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Rhodamine B base |
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| GT5360-100G | Glentham Life Sciences | 100 g | EUR 158 |
Rhodamine B base |
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| GT5360-10G | Glentham Life Sciences | 10 g | EUR 50 |
To the most effective for our data, that is the primary report on manufacturing of polyclonal antiserum utilizing recombinant virion-associated protein as fusion protein, which could possibly be used for screening CYMV-infected crops by ELISA and IC-PCR. These immunodiagnostic strategies might be successfully employed in routine indexing of citrus and in quarantine course of.
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