Mozzarella di Bufala Campana and Feta are two cheeses with Protected Designation of Origin the fraudulent adulteration of which with bovine milk should be routinely checked to make sure that shoppers really purchase these high-end merchandise and keep away from well being points associated to bovine milk allergy. Right here, we employed, for the primary time, a silicon-based photonic immunosensor for the detection of mozzarella and feta adulteration with bovine milk. The photonic immunosensor used depends on Mach-Zehnder interferometers monolithically built-in together with their respective gentle sources on a silicon chip. A rabbit polyclonal antiserum raised towards bovine κ-casein was used for the event of a aggressive immunoassay realized in three steps, together with a response with the antiserum, a biotinylated anti-rabbit IgG antibody, and streptavidin.
The implementation of this assay configuration considerably lowered the non-specific sign as a result of cheese matrix, and allowed completion of the assay in ∼9 min. After optimization of all assay situations, bovine cheese may very well be quantified in mozzarella or feta at concentrations as little as 0.5 and 0.25% (w/w), respectively; each quantification limits had been under the utmost allowable content material of bovine milk in mozzarella and feta (1% w/w) in line with the EU rules. Equally essential, the assays had been reproducible with intra- and inter-assay coefficients of variation <10%, and exhibited a large linear dynamic vary that prolonged as much as 50 and 25% (w/w) for mozzarella and feta, respectively. Taking into consideration its efficiency, the proposed immunosensor could also be reworked to a brand new device towards fraudulent actions within the dairy trade.
Immunological detection of pyrazine-2-carboxylic acid for the detection of pyrazinamide resistance in Mycobacterium tuberculosis
Pyrazinamide (PZA) susceptibility testing in Mycobacterium tuberculosis (Mtb) is a present space of growth and PZA-resistant strains are more and more prevalent. Earlier research have demonstrated that the detection of pyrazinoic acid (POA), the metabolite produced by the deamidation of PZA, is an efficient predictor for PZA resistance since a resistant pressure wouldn’t convert PZA into POA at a vital required fee, whereas a inclined pressure will do, expelling POA to the extracellular atmosphere at a sure fee, and permitting for quantification of this accrued analyte.
In an effort to quantify POA, an oblique aggressive ELISA (icELISA) take a look at utilizing hyperimmune polyclonal rabbit serum towards POA was developed: for this function, pure POA was first covalently linked to the extremely immunogenic Keyhole Limpet Hemocyanine, and inoculated in rabbits. A assemble fabricated from bovine serum albumin (BSA) linked to pure POA and glued on the backside of wells was used as a competitor towards spiked samples and liquid Mtb tradition supernatants. When spiked samples (business POA alone) had been analyzed, the half maximal inhibitory focus (IC50) was 1.16 mg/mL, the restrict of detection 200 μg/mL and the assay was particular (it didn’t detect PZA, IC50 > 20 mg/mL).
Nonetheless, tradition supernatants (7H9-OADC-PANTA medium) disrupted the competitors and a correct icELISA curve was not obtainable. We think about that, though now we have proven that it’s possible to induce antibodies towards POA, matrix results may harm its analytical usefulness; a number of, upcoming methods to unravel this impediment are instructed.
Macroporous Polymer Monoliths for Affinity Chromatography and Stable-Part Enzyme Processing
These days, monolithic stationary phases, due to their particular morphology and massive permeability, are broadly used for the event and realization of quick dynamic and static processes primarily based on the mass transition between liquid and strong phases. These are liquid chromatography, solid-phase synthesis, microarrays, flow-through enzyme reactors, and many others. Excessive-performance liquid chromatography on monoliths, together with the bioaffinity mode, represents distinctive method acceptable for quick and environment friendly separation of organic (macro)molecules of various configurations and dimensions (proteins, nucleic acids, peptides), in addition to such supramolecular methods as viruses.
Within the edited chapter, the examples of the appliance of commercially obtainable macroporous monoliths for contemporary affinity processing are introduced. Particularly, the unique strategies developed for environment friendly isolation and fractionation of monospecific antibodies from rabbit blood sera, the potential for simultaneous affinity separation of protein G and serum albumin from human serum, the isolation of recombinant merchandise, equivalent to protein G and tissue plasminogen activator, respectively, are described intimately. The instructed and realized multifunctional fractionation of polyclonal swimming pools of antibodies by the mixture of a number of brief monolithic columns (disks) with completely different affinity functionalities stacked in the identical cartridge represents the unique and virtually worthwhile methodology that can be utilized in biotechnology.
IgG Antibody (Rabbit anti Goat) (Fc) |
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| GWB-7BB68B | GenWay Biotech | 1 ml | Ask for price |
IgG Antibody (Rabbit anti Goat) (Fc) |
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| GWB-CBDFE9 | GenWay Biotech | 1.5 ml | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-39B790 | GenWay Biotech | 2 mg | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-3F03E0 | GenWay Biotech | 2 mg | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-423B57 | GenWay Biotech | 1 ml | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-4BF9D8 | GenWay Biotech | 10 mg | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-5F513F | GenWay Biotech | 1 mg | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-233373 | GenWay Biotech | 1.5 ml | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-10D6B2 | GenWay Biotech | 2 ml | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-1CDFA2 | GenWay Biotech | 0.5 mg | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-AB00ED | GenWay Biotech | 2 ml | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-AC3EDA | GenWay Biotech | 5 mg | Ask for price |
IgG Antibody (Goat anti Rabbit) (H&L) |
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| GWB-74EC69 | GenWay Biotech | 2 mg | Ask for price |
Rabbit Anti-Sheep/Goat IgG antibody |
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| STJ99581-100l | St John's Laboratory | 100 µl | EUR 104.4 |
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Description: Unconjugated Rabbit monoclonal to Sheep/Goat IgG antibody |
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Rabbit Anti-Sheep/Goat IgG antibody |
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| STJ99581-1mL | St John's Laboratory | 1 mL | EUR 546 |
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Description: Unconjugated Rabbit monoclonal to Sheep/Goat IgG antibody |
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IgG Antibody (Rabbit anti Goat) (H&L) |
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| GWB-681D85 | GenWay Biotech | 2 mg | Ask for price |
IgG Antibody (Rabbit anti Goat) (H&L) |
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| GWB-AFFF41 | GenWay Biotech | 1.5 mg | Ask for price |
Goat Anti Rabbit Igg (Fc) Polyclonal Antibody |
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| DPBT-67197GR | Creative Diagnostics | 0.5 mg | EUR 696 |
Goat Anti Rabbit Igg/Igm Polyclonal Antibody |
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| DPBT-67204GR | Creative Diagnostics | 1 mg | EUR 795.6 |
IgG Antibody (Goat anti Rabbit) F(ab )2 |
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| GWB-6B86BE | GenWay Biotech | 2 ml | Ask for price |
Goat anti Rabbit IgG |
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| 41C-CB1143 | Fitzgerald | 2 mg | EUR 259 |
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Description: Goat anti Rabbit IgG secondary antibody |
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Goat anti Rabbit IgG |
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| 41C-CB1150 | Fitzgerald | 10 mg | EUR 985 |
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Description: Goat anti Rabbit IgG secondary antibody |
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Goat anti Rabbit IgG |
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| 41R-1079 | Fitzgerald | 2 mg | EUR 127 |
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Description: Goat anti Rabbit IgG secondary antibody |
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Goat anti Rabbit IgG |
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| 40C-CB1157 | Fitzgerald | 5 mg | Ask for price |
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Description: Goat anti Rabbit IgG secondary antibody |
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Goat anti Rabbit IgG |
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| 41-GR30 | Fitzgerald | 10 mg | EUR 229 |
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Description: Goat anti Rabbit IgG secondary antibody |
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Goat anti Rabbit IgG |
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| 41-GR31 | Fitzgerald | 5 mg | EUR 194.4 |
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Description: Goat anti Rabbit IgG secondary antibody |
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Goat anti Rabbit IgG |
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| 40-GR30 | Fitzgerald | 100 ml | EUR 332 |
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Description: Goat anti Rabbit IgG secondary antibody |
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Goat anti Rabbit IgG |
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| 40-GR30S | Fitzgerald | 100 ml | EUR 95 |
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Description: Goat anti Rabbit IgG secondary antibody |
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goat Anti Rabbit IgG |
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| E61I01301 | EnoGene | 1mg | EUR 172.5 |
goat Anti Rabbit IgG |
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| MBS8503153-1mg | MyBiosource | 1mg | EUR 255 |
goat Anti Rabbit IgG |
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| MBS8503153-5x1mg | MyBiosource | 5x1mg | EUR 1000 |
GOAT ANTI RABBIT IgG |
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| MBS235217-1mg | MyBiosource | 1mg | EUR 245 |
GOAT ANTI RABBIT IgG |
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| MBS235217-5x1mg | MyBiosource | 5x1mg | EUR 925 |
Goat anti Rabbit IgG |
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| MBS539091-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Goat anti Rabbit IgG |
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| MBS539100-100mL | MyBiosource | 100mL | EUR 870 |
Goat anti Rabbit IgG |
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| MBS539100-5x100mL | MyBiosource | 5x100mL | EUR 3760 |
Goat anti Rabbit IgG |
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| MBS539169-10mg | MyBiosource | 10mg | EUR 605 |
Goat anti Rabbit IgG |
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| MBS539169-5x10mg | MyBiosource | 5x10mg | EUR 2570 |
Goat anti Rabbit IgG |
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| MBS539780-10mg | MyBiosource | 10mg | EUR 1695 |
Goat anti Rabbit IgG |
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| MBS539780-5x10mg | MyBiosource | 5x10mg | EUR 7465 |
Goat anti Rabbit IgG |
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| MBS539800-2mg | MyBiosource | 2mg | EUR 480 |
Goat anti Rabbit IgG |
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| MBS539800-5x2mg | MyBiosource | 5x2mg | EUR 2005 |
Goat anti Rabbit IgG Antibody (Fc) (Frag Specific) |
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| GWB-5B2214 | GenWay Biotech | 1.5 ml | Ask for price |
Goat Anti Rabbit Igg (H/L) Polyclonal Antibody |
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| CPBT-67353GR | Creative Diagnostics | 2 mg | EUR 696 |
Goat Anti Rabbit Igg/Igm Polyclonal Antibody,HRP |
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| DPBT-67203GR | Creative Diagnostics | 1 ml | EUR 858 |
Goat Anti Rabbit Igg/Igm Polyclonal Antibody,RPE |
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| DPBT-67205GR | Creative Diagnostics | 0.5 mg | EUR 1158 |
Rabbit anti Goat IgG |
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| 20-S5801R000-VO | Fitzgerald | 10 ml | EUR 95 |
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Description: Rabbit anti Goat IgG antibody |
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Rabbit anti Goat IgG |
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| 41C-CB0529 | Fitzgerald | 2 mg | EUR 234 |
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Description: Rabbit anti Goat IgG secondary antibody |
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Rabbit anti Goat IgG |
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| 40C-CB0536 | Fitzgerald | 1.5 mg | EUR 274 |
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Description: Rabbit anti Goat IgG secondary antibody |
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Rabbit anti Goat IgG |
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| E61I01002 | EnoGene | 1mg | EUR 225 |
Rabbit anti Goat IgG |
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| MBS539774-2mg | MyBiosource | 2mg | EUR 435 |
Rabbit anti Goat IgG |
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| MBS539774-5x2mg | MyBiosource | 5x2mg | EUR 1815 |
Rabbit Anti Goat Igg (Fc) Polyclonal Antibody |
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| DPBT-67069RG | Creative Diagnostics | 1 mg | EUR 795.6 |
Goat Anti Rabbit Igg/Igm Polyclonal Antibody,FITC |
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| DPBT-67202GR | Creative Diagnostics | 1 mg | EUR 852 |
Rabbit Anti Goat Igg (Fab') Polyclonal Antibody |
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| DPBT-67090RG | Creative Diagnostics | 0.5 mg | EUR 696 |
Goat Anti Rabbit Igg/Igm Polyclonal Antibody,TRITC |
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| DPBT-67207GR | Creative Diagnostics | 1 mg | EUR 795.6 |
Goat Anti Rabbit Igg/Igm Polyclonal Antibody,Biotin |
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| DPBT-67201GR | Creative Diagnostics | 1 mg | EUR 852 |
Rabbit Anti-Sheep / Goat IgG (H&L) Antibody |
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| 20-abx134906 | Abbexa |
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Rabbit Anti-Sheep / Goat IgG H&L Antibody |
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| abx134906-01mg | Abbexa | 0.1 mg | EUR 187.5 |
Goat anti Rabbit IgG Fc |
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| 41C-CB1136 | Fitzgerald | 2 mg | EUR 234 |
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Description: Goat anti Rabbit IgG secondary antibody |
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Goat anti Rabbit IgG Fc |
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| 70R-IG011 | Fitzgerald | 1.5 mg | EUR 348 |
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Description: Goat anti Rabbit IgG Fc secondary antibody |
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Goat anti Rabbit IgG Fc |
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| 70R-IG017 | Fitzgerald | 2 mg | EUR 347 |
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Description: Goat anti Rabbit IgG Fc secondary antibody |
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GOAT ANTI RABBIT IgG (Fc) |
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| MBS235249-1mg | MyBiosource | 1mg | EUR 265 |
GOAT ANTI RABBIT IgG (Fc) |
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| MBS235249-5x1mg | MyBiosource | 5x1mg | EUR 1020 |
Goat anti Rabbit IgG (Fc) |
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| MBS316567-2mL | MyBiosource | 2mL | EUR 590 |
Goat anti Rabbit IgG (Fc) |
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| MBS316567-5x2mL | MyBiosource | 5x2mL | EUR 2485 |
Goat anti Rabbit IgG (Fc) |
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| MBS316619-2mg | MyBiosource | 2mg | EUR 295 |
Goat anti Rabbit IgG (Fc) |
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| MBS316619-5x2mg | MyBiosource | 5x2mg | EUR 1150 |
Goat anti Rabbit IgG Fc |
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| MBS539776-2mg | MyBiosource | 2mg | EUR 435 |
Goat anti Rabbit IgG Fc |
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| MBS539776-5x2mg | MyBiosource | 5x2mg | EUR 1815 |
Goat anti Rabbit IgG Fc |
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| MBS534059-2mg | MyBiosource | 2mg | EUR 485 |
Goat anti Rabbit IgG Fc |
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| MBS534059-5x2mg | MyBiosource | 5x2mg | EUR 2030 |
Goat anti Rabbit IgG Fc |
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| MBS535045-15mg | MyBiosource | 1.5mg | EUR 490 |
Goat anti Rabbit IgG Fc |
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| MBS535045-5x15mg | MyBiosource | 5x1.5mg | EUR 2055 |
Goat anti Rabbit IgG (HRP) |
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| 43C-CB1141 | Fitzgerald | 2 mg | EUR 397 |
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Description: Goat anti Rabbit IgG secondary antibody (HRP) |
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Goat anti Rabbit IgG (HRP) |
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| 43C-CB1148 | Fitzgerald | 2 mg | EUR 353 |
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Description: Goat anti Rabbit IgG secondary antibody (HRP) |
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Goat anti Rabbit IgG (HRP) |
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| 43C-CB1155 | Fitzgerald | 2 mg | EUR 344 |
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Description: Goat anti Rabbit IgG secondary antibody (HRP) |
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Goat anti Rabbit IgG (HRP) |
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| 43C-CB1162 | Fitzgerald | 2 mg | EUR 376 |
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Description: Goat anti Rabbit IgG secondary antibody (HRP) |
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Goat anti Rabbit IgG (HRP) |
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| 43R-1495 | Fitzgerald | 1 mg | EUR 115 |
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Description: Goat anti Rabbit IgG secondary antibody (HRP) |
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Goat anti Rabbit IgG Fab |
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| MBS573197-1mL | MyBiosource | 1mL | EUR 365 |
Goat anti Rabbit IgG Fab |
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| MBS573197-5x1mL | MyBiosource | 5x1mL | EUR 1490 |
Goat anti Rabbit IgG (HRP) |
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| MBS538611-2mg | MyBiosource | 2mg | EUR 620 |
Goat anti Rabbit IgG (HRP) |
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| MBS538611-5x2mg | MyBiosource | 5x2mg | EUR 2635 |
Goat anti Rabbit IgG (HRP) |
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| MBS538619-2mg | MyBiosource | 2mg | EUR 655 |
Goat anti Rabbit IgG (HRP) |
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| MBS538619-5x2mg | MyBiosource | 5x2mg | EUR 2805 |
Goat anti Rabbit IgG (HRP) |
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| MBS539812-2mg | MyBiosource | 2mg | EUR 595 |
Goat anti Rabbit IgG (HRP) |
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| MBS539812-5x2mg | MyBiosource | 5x2mg | EUR 2515 |
Goat anti Rabbit IgG (HRP) |
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| MBS539843-2mg | MyBiosource | 2mg | EUR 715 |
Goat anti Rabbit IgG (HRP) |
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| MBS539843-5x2mg | MyBiosource | 5x2mg | EUR 3075 |
Goat anti Rabbit IgG (FITC) |
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| 43C-CB1137 | Fitzgerald | 2 mg | EUR 256 |
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Description: Goat anti Rabbit IgG secondary antibody (FITC) |
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Goat anti Rabbit IgG (FITC) |
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| 43C-CB1144 | Fitzgerald | 2 mg | EUR 293 |
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Description: Goat anti Rabbit IgG secondary antibody (FITC) |
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Goat anti Rabbit IgG (FITC) |
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| 43C-CB1158 | Fitzgerald | 2 mg | EUR 418 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
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Goat anti Rabbit IgG (FITC) |
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| 43R-1494 | Fitzgerald | 2 mg | EUR 176 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
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Goat anti Rabbit IgG (FITC) |
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| 43-GR59 | Fitzgerald | 2 mg | EUR 276 |
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Description: Goat anti Rabbit IgG secondary antibody (FITC) |
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Goat anti Rabbit IgG (FITC) |
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| MBS538607-2mg | MyBiosource | 2mg | EUR 540 |
Goat anti Rabbit IgG (FITC) |
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| MBS538607-5x2mg | MyBiosource | 5x2mg | EUR 2280 |
Goat anti Rabbit IgG (FITC) |
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| MBS538616-2mg | MyBiosource | 2mg | EUR 750 |
Goat anti Rabbit IgG (FITC) |
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| MBS538616-5x2mg | MyBiosource | 5x2mg | EUR 3230 |
Goat anti Rabbit IgG (FITC) |
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| MBS539784-2mg | MyBiosource | 2mg | EUR 480 |
Goat anti Rabbit IgG (FITC) |
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| MBS539784-5x2mg | MyBiosource | 5x2mg | EUR 2005 |
Goat anti Rabbit IgG (FITC) |
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| MBS539825-2mg | MyBiosource | 2mg | EUR 500 |
Goat anti Rabbit IgG (FITC) |
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| MBS539825-5x2mg | MyBiosource | 5x2mg | EUR 2105 |
goat Anti Rabbit IgG-HRP |
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| E61I01303 | EnoGene | 1mg | EUR 345 |
Goat anti Rabbit IgG (biotin) |
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| 43C-CB1140 | Fitzgerald | 2 mg | EUR 297 |
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Description: Goat anti Rabbit IgG secondary antibody (biotin) |
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Goat anti Rabbit IgG (biotin) |
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| 43C-CB1147 | Fitzgerald | 2 mg | EUR 373 |
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Description: Goat anti Rabbit IgG secondary antibody (biotin) |
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Goat anti Rabbit IgG (biotin) |
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| 43C-CB1154 | Fitzgerald | 2 mg | EUR 297 |
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Description: Goat anti Rabbit IgG secondary antibody (biotin) |
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Goat anti Rabbit IgG (biotin) |
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| 43C-CB1161 | Fitzgerald | 2 mg | EUR 376 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
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Goat anti Rabbit IgG (biotin) |
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| 43R-1493 | Fitzgerald | 2 mg | EUR 167 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
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goat Anti Rabbit IgG-FITC |
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| E61I01304 | EnoGene | 1mg | EUR 431.25 |
goat Anti Rabbit IgG-FITC |
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| MBS8502220-1mg | MyBiosource | 1mg | EUR 510 |
goat Anti Rabbit IgG-FITC |
|||
| MBS8502220-5x1mg | MyBiosource | 5x1mg | EUR 2140 |
Goat anti Rabbit IgG (biotin) |
|||
| MBS538610-2mg | MyBiosource | 2mg | EUR 655 |
Goat anti Rabbit IgG (biotin) |
|||
| MBS538610-5x2mg | MyBiosource | 5x2mg | EUR 2805 |
Goat anti Rabbit IgG (biotin) |
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| MBS538618-2mg | MyBiosource | 2mg | EUR 655 |
Goat anti Rabbit IgG (biotin) |
|||
| MBS538618-5x2mg | MyBiosource | 5x2mg | EUR 2805 |
Goat anti Rabbit IgG (biotin) |
|||
| MBS539045-2mg | MyBiosource | 2mg | EUR 540 |
Goat anti Rabbit IgG (biotin) |
|||
| MBS539045-5x2mg | MyBiosource | 5x2mg | EUR 2280 |
Goat anti Rabbit IgG (biotin) |
|||
| MBS539773-2mg | MyBiosource | 2mg | EUR 540 |
Goat anti Rabbit IgG (biotin) |
|||
| MBS539773-5x2mg | MyBiosource | 5x2mg | EUR 2280 |
goat Anti Rabbit IgG-Biotin |
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| E61I01302 | EnoGene | 1mg | EUR 345 |
goat Anti Rabbit IgG-Biotin |
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| MBS8502571-1mg | MyBiosource | 1mg | EUR 425 |
goat Anti Rabbit IgG-Biotin |
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| MBS8502571-5x1mg | MyBiosource | 5x1mg | EUR 1755 |
Goat Anti Rabbit Igg (Fab') (H/L) Polyclonal Antibody |
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| DPBT-67196GR | Creative Diagnostics | 0.5 mg | EUR 696 |
Goat anti Rabbit IgG (rhodamine) |
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| 43C-CB1138 | Fitzgerald | 2 mg | EUR 256 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (rhodamine) |
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| 43C-CB1145 | Fitzgerald | 2 mg | EUR 352 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
|||
Goat anti Rabbit IgG (rhodamine) |
|||
| 43C-CB1152 | Fitzgerald | 2 mg | EUR 265 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
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Goat anti Rabbit IgG (rhodamine) |
|||
| 43C-CB1159 | Fitzgerald | 2 mg | EUR 418 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
|||
Goat anti Rabbit IgG (rhodamine) |
|||
| MBS538605-2mg | MyBiosource | 2mg | EUR 480 |
Goat anti Rabbit IgG (rhodamine) |
|||
| MBS538605-5x2mg | MyBiosource | 5x2mg | EUR 2005 |
Goat anti Rabbit IgG (rhodamine) |
|||
| MBS538608-2mg | MyBiosource | 2mg | EUR 660 |
Goat anti Rabbit IgG (rhodamine) |
|||
| MBS538608-5x2mg | MyBiosource | 5x2mg | EUR 2825 |
Goat anti Rabbit IgG (rhodamine) |
|||
| MBS538613-2mg | MyBiosource | 2mg | EUR 480 |
As well as, macroporous monoliths had been tailored to the immobilization of such completely different enzymes as polynucleotide phosphorylase, ribonuclease A, α-chymotrypsin, chitinolytic biocatalysts, β-xylosidase, and β-xylanase. The opportunity of use of immobilized enzyme reactors primarily based on monoliths for various functions is demonstrated.
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