An immunobiosensor assay was developed for multi-residue screening in bovine milk of the mother or father amphenicols, thiamphenicol and florfenicol, together with the metabolite florfenicol amine. A polyclonal antibody raised in a rabbit after immunisation with a florfenicol amine-protein conjugate was employed within the assay. Milk samples had been subjected to acetonitrile extraction, reconstituted in buffer and diluted previous to biosensor evaluation. Validation knowledge obtained from the evaluation of fortified samples has proven that the tactic has a detection functionality of lower than 0.25 µg kg-1 for florfenicol and fewer than 0.5 µg kg-1 for florfenicol amine and thiamphenicol.
The cross-reactivity profile and validation knowledge for the detection of those amphenicols is offered along with outcomes obtained following the evaluation of florfenicol incurred samples utilizing the developed screening technique together with a comparability of outcomes obtained from the evaluation of the identical incurred samples utilizing an MRM3 UPLC-MS/MS confirmatory technique. Outcomes are additionally offered obtained from the evaluation of samples from each handled and non-treated animals which had been co-housed and which present the potential for cross-contamination. Underneath optimum circumstances, the potentiometric immunosensor exhibited good potentiometric responses for the detection of cTcI and allowed the dedication of the goal analyte at a focus as little as 7.Three pg mL-1.
An intermediate precision of ≤8.7% was completed with batch-to-batch identification. In the meantime, the potentiometric immunosensor confirmed good anti-interfering capability and selectivity towards different proteins and biomarkers. Importantly, our system displayed excessive accuracy for the evaluation of human plasma serum samples containing goal cTcI relative to business human cTcI enzyme-linked immunosorbent assay (ELISA) kits.
Neutralizing efficiency and immunochemical analysis of an anti-Crotalus mictlantecuhtli experimental serum
Snakebite in Mexico is often handled with an antivenom which makes use of Bothrops asper and Crotalus simus venoms as immunogens. Present taxonomic suggestions for the C. simus species advanced counsel a novel endemic species from Mexico: Crotalus mictlantecuhtli. The intention of this report was to judge the immunogenic properties of C. mictlantecuhtli venom and its potential to generate polyclonal antibodies able to neutralizing different pitviper venoms. We generated an experimental anti-Crotalus mictlantecuhtli serum, utilizing the rabbit mannequin, to check recognition and neutralizing capability towards the homologous venom in addition to venoms from C. atrox, C.basiliscus, C. durissus terrificus, C. scutulatus salvini, C. tzabcan and Ophryacus sphenophrys.
Pre-incubation neutralization experiments utilizing our experimental serum confirmed optimistic outcomes towards venoms containing crotoxin, whereas venoms from two non-neurotoxic pit-vipers weren’t neutralized. Rescue experiments in mice confirmed that, when intravenously injected (i.v.), C. mictlantecuhtli venom just isn’t neutralized by a most dose of Antivipmyn® and the experimental serum after 5 min of envenomation, albeit mice envenomated intraperitoneally (i.p.) and rescued i.v. with Antivipmyn® survived even at 50 min after envenomation. Our outcomes spotlight the significance of utilizing the extremely neurotoxic C. mictlantecuhtli venom to extend antivenom effectiveness towards Mexican neurotoxic pitvipers. Preeclampsia (PE) and human immunodeficiency virus (HIV) have been linked with marked will increase in maternal stress, leading to a major change in placental perform starting from alterations in placental construction to the exact and delicate transformations in placental gene expression.
Such adjustments might result in altered transport of important indicators to the fetus, which might have long-term impacts on offspring well being and consequently have an effect on fetal neurodevelopment. Subsequently, this work investigated the position of placental 11β-hydroxysteroid dehydrogenase sorts 2 (11β-HSD2) in HIV related preeclampsia. The placenta had been obtained from 76 pregnant ladies, which had been stratified primarily based on being pregnant sort and HIV standing into; Normotensive HIV unfavorable, normotensive HIV optimistic, PE HIV unfavorable and PE HIV optimistic.
A Novel KRAS Antibody Highlights a Regulation Mechanism of Submit-Translational Modifications of KRAS throughout Tumorigenesis
KRAS is a strong oncogene answerable for the event of many cancers. Regardless of the nice progress in understanding its perform over the last decade, the research of KRAS expression, subcellular localization, and post-translational modifications stays technically difficult. Accordingly, many aspects of KRAS biology are nonetheless unknown. Antibodies might be an efficient and easy-to-use device for in vitro and in vivo analysis on KRAS.
Right here, we generated a novel rabbit polyclonal antibody that permits immunolabeling of cells and tissues overexpressing KRAS. Cell transfection experiments with expression vectors for the members of the RAS household revealed a preferential specificity of this antibody for KRAS. As well as, KRAS was sensitively detected in a mouse tissue electroporated with an expression vector. Apparently, our antibody was in a position to detect endogenous types of unprenylated (immature) and prenylated (mature) KRAS in mouse organs. We discovered that KRAS prenylation was elevated ex vivo and in vivo in a mannequin of KRASG12D-driven tumorigenesis, which was concomitant with an induction of expression of important KRAS prenylation enzymes.
 Polyclonal ASAH2 Antibody |
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| APG02011G | Leading Biology | 0.1mg | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ASAH2 . This antibody is tested and proven to work in the following applications: |
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 ASAH2 Polyclonal Antibody |
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| A62106 | EpiGentek | 100 µg | EUR 570.55 |
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Description: fast delivery possible |
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 ASAH2 Rabbit pAb |
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| A12596-100ul | Abclonal | 100 ul | EUR 308 |
ASAH2 Rabbit pAb |
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| A12596-200ul | Abclonal | 200 ul | EUR 459 |
ASAH2 Rabbit pAb |
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| A12596-20ul | Abclonal | 20 ul | EUR 183 |
ASAH2 Rabbit pAb |
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| A12596-50ul | Abclonal | 50 ul | EUR 223 |
ASAH2 Rabbit pAb |
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| A7985-100ul | Abclonal | 100 ul | EUR 308 |
ASAH2 Rabbit pAb |
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| A7985-200ul | Abclonal | 200 ul | EUR 459 |
ASAH2 Rabbit pAb |
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| A7985-20ul | Abclonal | 20 ul | EUR 183 |
ASAH2 Rabbit pAb |
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| A7985-50ul | Abclonal | 50 ul | EUR 223 |
 ASAH2 Antibody |
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| 24731-100ul | SAB | 100ul | EUR 390 |
 ASAH2 antibody |
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| 70R-12124 | Fitzgerald | 100 ug | EUR 403 |
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Description: Rabbit polyclonal ASAH2 antibody |
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ASAH2 Antibody |
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| 36240-100ul | SAB | 100ul | EUR 252 |
ASAH2 Antibody |
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| 3828-100 | Biovision | EUR 316 | |
ASAH2 Antibody |
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| 3828-30T | Biovision | EUR 146 | |
ASAH2 Antibody |
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| 1-CSB-PA002170EA01HU | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
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ASAH2 Antibody |
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| 1-CSB-PA076840 | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
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ASAH2 Antibody |
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| 1-CSB-PA133826 | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
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 ASAH2 Polyclonal Antibody, HRP Conjugated |
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| A62107 | EpiGentek | 100 µg | EUR 570.55 |
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Description: reagents widely cited |
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 ASAH2 Polyclonal Antibody, FITC Conjugated |
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| A62108 | EpiGentek | 100 µg | EUR 570.55 |
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Description: Ask the seller for details |
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 ASAH2 Polyclonal Antibody, Biotin Conjugated |
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| A62109 | EpiGentek | 100 µg | EUR 570.55 |
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Description: The best epigenetics products |
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 ASAH2 Conjugated Antibody |
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| C36240 | SAB | 100ul | EUR 397 |
 Anti-ASAH2 antibody |
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| STJ110292 | St John's Laboratory | 100 µl | EUR 277 |
Anti-ASAH2 antibody |
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| STJ114470 | St John's Laboratory | 100 µl | EUR 277 |
 ASAH2 siRNA |
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| 20-abx900469 | Abbexa |
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ASAH2 siRNA |
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| 20-abx908269 | Abbexa |
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ASAH2 siRNA |
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| 20-abx908270 | Abbexa |
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 ASAH2 Antibody, HRP conjugated |
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| 1-CSB-PA002170EB01HU | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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 ASAH2 Antibody, FITC conjugated |
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| 1-CSB-PA002170EC01HU | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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 ASAH2 Antibody, Biotin conjugated |
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| 1-CSB-PA002170ED01HU | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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 ASAH2 Blocking Peptide |
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| 33R-10941 | Fitzgerald | 50 ug | EUR 191 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ASAH2 antibody, catalog no. 70R-12124 |
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ASAH2 Blocking Peptide |
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| 3828BP-50 | Biovision | EUR 153 | |
 ASAH2 cloning plasmid |
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| CSB-CL865115HU-10ug | Cusabio | 10ug | EUR 474 |
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Description: A cloning plasmid for the ASAH2 gene. |
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 Mouse ASAH2 shRNA Plasmid |
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| 20-abx974501 | Abbexa |
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 Rat ASAH2 shRNA Plasmid |
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| 20-abx987160 | Abbexa |
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 Human ASAH2 shRNA Plasmid |
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| 20-abx961153 | Abbexa |
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) ASAH2 Recombinant Protein (Human) |
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| RP036760 | ABM | 100 ug | Ask for price |
) ASAH2 Recombinant Protein (Mouse) |
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| RP117410 | ABM | 100 ug | Ask for price |
) ASAH2 Recombinant Protein (Rat) |
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| RP191108 | ABM | 100 ug | Ask for price |
 Antibody) N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx007107 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| abx025039-400ul | Abbexa | 400 ul | EUR 523 |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| abx025039-80l | Abbexa | 80 µl | EUR 286 |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx214010 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx214011 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx177710 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx173712 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx303129 | Abbexa |
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 Antibody (HRP)) N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (HRP) |
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| 20-abx303130 | Abbexa |
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 Antibody (FITC)) N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (FITC) |
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| 20-abx303131 | Abbexa |
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 Antibody (Biotin)) N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (Biotin) |
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| 20-abx303132 | Abbexa |
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 (pORF)) Asah2 ORF Vector (Rat) (pORF) |
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| ORF063704 | ABM | 1.0 ug DNA | EUR 506 |
 (pORF)) ASAH2 ORF Vector (Human) (pORF) |
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| ORF012254 | ABM | 1.0 ug DNA | EUR 354 |
 (pORF)) Asah2 ORF Vector (Mouse) (pORF) |
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| ORF039138 | ABM | 1.0 ug DNA | EUR 506 |
 (OKCD01831)) ASAH2 ELISA Kit (Human) (OKCD01831) |
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| OKCD01831 | Aviva Systems Biology | 96 Wells | EUR 831 |
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Description: Description of target: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.31 ng/mL |
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) Asah2 sgRNA CRISPR Lentivector set (Rat) |
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| K7087001 | ABM | 3 x 1.0 ug | EUR 339 |
) ASAH2 sgRNA CRISPR Lentivector set (Human) |
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| K0130501 | ABM | 3 x 1.0 ug | EUR 339 |
) Asah2 sgRNA CRISPR Lentivector set (Mouse) |
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| K3499601 | ABM | 3 x 1.0 ug | EUR 339 |
 Protein) Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) Protein |
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| 20-abx654471 | Abbexa |
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 ELISA Kit (CUSTOM)) Mouse ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
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| ELI-34287m | Lifescience Market | 96 Tests | EUR 865 |
 ELISA Kit (CUSTOM)) Human ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
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| ELI-34330h | Lifescience Market | 96 Tests | EUR 824 |
 ELISA Kit (CUSTOM)) Rat ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
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| ELI-48960r | Lifescience Market | 96 Tests | EUR 886 |
 (Target 1)) Asah2 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K7087002 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) Asah2 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K7087003 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) Asah2 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K7087004 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 1)) ASAH2 sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K0130502 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) ASAH2 sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K0130503 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) ASAH2 sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K0130504 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 1)) Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K3499602 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K3499603 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K3499604 | ABM | 1.0 ug DNA | EUR 154 |
 (pPB-C-His)) ASAH2 Protein Vector (Mouse) (pPB-C-His) |
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| PV156550 | ABM | 500 ng | EUR 1065 |
 (pPB-N-His)) ASAH2 Protein Vector (Mouse) (pPB-N-His) |
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| PV156551 | ABM | 500 ng | EUR 1065 |
 (pPM-C-HA)) ASAH2 Protein Vector (Mouse) (pPM-C-HA) |
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| PV156552 | ABM | 500 ng | EUR 1065 |
 (pPM-C-His)) ASAH2 Protein Vector (Mouse) (pPM-C-His) |
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| PV156553 | ABM | 500 ng | EUR 1065 |
 (pPB-C-His)) ASAH2 Protein Vector (Rat) (pPB-C-His) |
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| PV254814 | ABM | 500 ng | EUR 1166 |
 (pPB-N-His)) ASAH2 Protein Vector (Rat) (pPB-N-His) |
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| PV254815 | ABM | 500 ng | EUR 1166 |
 (pPM-C-HA)) ASAH2 Protein Vector (Rat) (pPM-C-HA) |
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| PV254816 | ABM | 500 ng | EUR 1166 |
 (pPM-C-His)) ASAH2 Protein Vector (Rat) (pPM-C-His) |
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| PV254817 | ABM | 500 ng | EUR 1166 |
 (pPB-His-MBP)) ASAH2 Protein Vector (Human) (pPB-His-MBP) |
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| PV324138 | ABM | 500 ng | EUR 481 |
 (pPB-His-GST)) ASAH2 Protein Vector (Human) (pPB-His-GST) |
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| PV324139 | ABM | 500 ng | EUR 481 |
 (pPB-C-His)) ASAH2 Protein Vector (Human) (pPB-C-His) |
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| PV049013 | ABM | 500 ng | EUR 481 |
 (pPB-N-His)) ASAH2 Protein Vector (Human) (pPB-N-His) |
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| PV049014 | ABM | 500 ng | EUR 481 |
 (pPM-C-HA)) ASAH2 Protein Vector (Human) (pPM-C-HA) |
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| PV049015 | ABM | 500 ng | EUR 481 |
 (pPM-C-His)) ASAH2 Protein Vector (Human) (pPM-C-His) |
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| PV049016 | ABM | 500 ng | EUR 329 |
 Asah2 3'UTR GFP Stable Cell Line |
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| TU152183 | ABM | 1.0 ml | Ask for price |
 Asah2 3'UTR Luciferase Stable Cell Line |
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| TU102183 | ABM | 1.0 ml | Ask for price |
Asah2 3'UTR Luciferase Stable Cell Line |
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| TU200951 | ABM | 1.0 ml | Ask for price |
Asah2 3'UTR GFP Stable Cell Line |
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| TU250951 | ABM | 1.0 ml | Ask for price |
 ASAH2 3'UTR GFP Stable Cell Line |
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| TU051220 | ABM | 1.0 ml | EUR 1521 |
 ASAH2 3'UTR Luciferase Stable Cell Line |
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| TU001220 | ABM | 1.0 ml | EUR 1521 |
 ELISA Kit) Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| 20-abx156834 | Abbexa |
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 CLIA Kit) Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) CLIA Kit |
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| 20-abx494694 | Abbexa |
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 (CMV) (pLenti-GIII-CMV)) ASAH2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV656977 | ABM | 1.0 ug DNA | EUR 1355 |
 (UbC) (pLenti-GIII-UbC)) ASAH2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV656981 | ABM | 1.0 ug DNA | EUR 1355 |
 (EF1a) (pLenti-GIII-EF1a)) ASAH2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV656982 | ABM | 1.0 ug DNA | EUR 1355 |
ELISA Kit) Human N-Acylsphingosine Amidohydrolase 2(ASAH2)ELISA Kit |
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| QY-E04735 | Qayee Biotechnology | 96T | EUR 361 |
Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| SEE689Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 4731.5 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
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Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| SEE689Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 477.3 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
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Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| SEE689Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 639 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
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Subsequently, our device helped us to place the sunshine on new rules of KRAS activation throughout most cancers initiation. The usage of this device by the RAS group may contribute to discovering novel points of KRAS biology.
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