Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor

Snakebite in Mexico is often handled with an antivenom which makes use of Bothrops asper and Crotalus simus venoms as immunogens. Present taxonomic suggestions for the C. simus species advanced counsel a novel endemic species from Mexico: Crotalus mictlantecuhtli. The intention of this report was to judge the immunogenic properties of C. mictlantecuhtli venom and its potential to generate polyclonal antibodies able to neutralizing different pitviper venoms. We generated an experimental anti-Crotalus mictlantecuhtli serum, utilizing the rabbit mannequin, to check recognition and neutralizing capability towards the homologous venom in addition to venoms from C. atrox, C.basiliscus, C. durissus terrificus, C. scutulatus salvini, C. tzabcan and Ophryacus sphenophrys.

Pre-incubation neutralization experiments utilizing our experimental serum confirmed optimistic outcomes towards venoms containing crotoxin, whereas venoms from two non-neurotoxic pit-vipers weren’t neutralized. Rescue experiments in mice confirmed that, when intravenously injected (i.v.), C. mictlantecuhtli venom just isn’t neutralized by a most dose of Antivipmyn® and the experimental serum after 5 min of envenomation, albeit mice envenomated intraperitoneally (i.p.) and rescued i.v. with Antivipmyn® survived even at 50 min after envenomation. Our outcomes spotlight the significance of utilizing the extremely neurotoxic C. mictlantecuhtli venom to extend antivenom effectiveness towards Mexican neurotoxic pitvipers. Preeclampsia (PE) and human immunodeficiency virus (HIV) have been linked with marked will increase in maternal stress, leading to a major change in placental perform starting from alterations in placental construction to the exact and delicate transformations in placental gene expression.

Such adjustments might result in altered transport of important indicators to the fetus, which might have long-term impacts on offspring well being and consequently have an effect on fetal neurodevelopment. Subsequently, this work investigated the position of placental 11β-hydroxysteroid dehydrogenase sorts 2 (11β-HSD2) in HIV related preeclampsia. The placenta had been obtained from 76 pregnant ladies, which had been stratified primarily based on being pregnant sort and HIV standing into; Normotensive HIV unfavorable, normotensive HIV optimistic, PE HIV unfavorable and PE HIV optimistic.

KRAS is a strong oncogene answerable for the event of many cancers. Regardless of the nice progress in understanding its perform over the last decade, the research of KRAS expression, subcellular localization, and post-translational modifications stays technically difficult. Accordingly, many aspects of KRAS biology are nonetheless unknown. Antibodies might be an efficient and easy-to-use device for in vitro and in vivo analysis on KRAS.

Right here, we generated a novel rabbit polyclonal antibody that permits immunolabeling of cells and tissues overexpressing KRAS. Cell transfection experiments with expression vectors for the members of the RAS household revealed a preferential specificity of this antibody for KRAS. As well as, KRAS was sensitively detected in a mouse tissue electroporated with an expression vector. Apparently, our antibody was in a position to detect endogenous types of unprenylated (immature) and prenylated (mature) KRAS in mouse organs. We discovered that KRAS prenylation was elevated ex vivo and in vivo in a mannequin of KRAS^G12D-driven tumorigenesis, which was concomitant with an induction of expression of important KRAS prenylation enzymes.

Polyclonal ASAH2 Antibody
APG02011G	Leading Biology	0.1mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ASAH2 . This antibody is tested and proven to work in the following applications:

ASAH2 Rabbit pAb
A12596-100ul	Abclonal	100 ul	EUR 308

ASAH2 Rabbit pAb
A12596-200ul	Abclonal	200 ul	EUR 459

ASAH2 Rabbit pAb
A12596-20ul	Abclonal	20 ul	EUR 183

ASAH2 Rabbit pAb
A12596-50ul	Abclonal	50 ul	EUR 223

ASAH2 Rabbit pAb
A7985-100ul	Abclonal	100 ul	EUR 308

ASAH2 Rabbit pAb
A7985-200ul	Abclonal	200 ul	EUR 459

ASAH2 Rabbit pAb
A7985-20ul	Abclonal	20 ul	EUR 183

ASAH2 Rabbit pAb
A7985-50ul	Abclonal	50 ul	EUR 223

ASAH2 Antibody
24731-100ul	SAB	100ul	EUR 390

ASAH2 Antibody
36240-100ul	SAB	100ul	EUR 252

ASAH2 Antibody
1-CSB-PA076840	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

ASAH2 Antibody
1-CSB-PA133826	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

ASAH2 Antibody
1-CSB-PA002170EA01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500

ASAH2 Antibody
3828-100	Biovision		EUR 316

ASAH2 Antibody
3828-30T	Biovision		EUR 146

ASAH2 antibody
70R-12124	Fitzgerald	100 ug	EUR 403
Description: Rabbit polyclonal ASAH2 antibody

ASAH2 Antibody
4743-002mg	ProSci	0.02 mg	EUR 171.82
Description: ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

ASAH2 Antibody
4743-01mg	ProSci	0.1 mg	EUR 436.42
Description: ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

ASAH2 Polyclonal Antibody, HRP Conjugated
A62107	EpiGentek	100 µg	EUR 570.55
Description: reagents widely cited

ASAH2 Polyclonal Antibody, FITC Conjugated
A62108	EpiGentek	100 µg	EUR 570.55
Description: Ask the seller for details

ASAH2 Polyclonal Antibody, Biotin Conjugated
A62109	EpiGentek	100 µg	EUR 570.55
Description: The best epigenetics products

Anti-ASAH2 antibody
STJ110292	St John's Laboratory	100 µl	EUR 277

Anti-ASAH2 antibody
STJ114470	St John's Laboratory	100 µl	EUR 277

ASAH2 Conjugated Antibody
C36240	SAB	100ul	EUR 397

ASAH2 siRNA
20-abx900469	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

ASAH2 siRNA
20-abx908269	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

ASAH2 siRNA
20-abx908270	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

ASAH2 Peptide
4743P	ProSci	0.05 mg	EUR 164.75
Description: (CT) ASAH2 peptide

ASAH2 Antibody, HRP conjugated
1-CSB-PA002170EB01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ASAH2 Antibody, FITC conjugated
1-CSB-PA002170EC01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ASAH2 Antibody, Biotin conjugated
1-CSB-PA002170ED01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

ASAH2 cloning plasmid
CSB-CL865115HU-10ug	Cusabio	10ug	EUR 474
Description: A cloning plasmid for the ASAH2 gene.

ASAH2 Blocking Peptide
3828BP-50	Biovision		EUR 153

ASAH2 Blocking Peptide
33R-10941	Fitzgerald	50 ug	EUR 191
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ASAH2 antibody, catalog no. 70R-12124

Mouse ASAH2 shRNA Plasmid
20-abx974501	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Rat ASAH2 shRNA Plasmid
20-abx987160	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Human ASAH2 shRNA Plasmid
20-abx961153	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

ASAH2 Recombinant Protein (Mouse)
RP117410	ABM	100 ug	Ask for price

ASAH2 Recombinant Protein (Rat)
RP191108	ABM	100 ug	Ask for price

ASAH2 Recombinant Protein (Human)
RP036760	ABM	100 ug	Ask for price

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody
20-abx303129	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody
20-abx007107	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody
abx025039-400ul	Abbexa	400 ul	EUR 523

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody
abx025039-80l	Abbexa	80 µl	EUR 286

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody
20-abx214010	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody
20-abx214011	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody
20-abx177710	Abbexa	EUR 1205.00 EUR 578.00	1 mg 200 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody
20-abx173712	Abbexa	EUR 857.00 EUR 439.00	1 mg 200 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (HRP)
20-abx303130	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (FITC)
20-abx303131	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (Biotin)
20-abx303132	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

ASAH2 ELISA Kit (Human) (OKCD01831)
OKCD01831	Aviva Systems Biology	96 Wells	EUR 831
Description: Description of target: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.31 ng/mL

ASAH2 ORF Vector (Human) (pORF)
ORF012254	ABM	1.0 ug DNA	EUR 354

Asah2 ORF Vector (Rat) (pORF)
ORF063704	ABM	1.0 ug DNA	EUR 506

Asah2 ORF Vector (Mouse) (pORF)
ORF039138	ABM	1.0 ug DNA	EUR 506

Asah2 sgRNA CRISPR Lentivector set (Mouse)
K3499601	ABM	3 x 1.0 ug	EUR 339

ASAH2 sgRNA CRISPR Lentivector set (Human)
K0130501	ABM	3 x 1.0 ug	EUR 339

Asah2 sgRNA CRISPR Lentivector set (Rat)
K7087001	ABM	3 x 1.0 ug	EUR 339

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) Protein
20-abx654471	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug

Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3499602	ABM	1.0 ug DNA	EUR 154

Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3499603	ABM	1.0 ug DNA	EUR 154

Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3499604	ABM	1.0 ug DNA	EUR 154

ASAH2 sgRNA CRISPR Lentivector (Human) (Target 1)
K0130502	ABM	1.0 ug DNA	EUR 154

ASAH2 sgRNA CRISPR Lentivector (Human) (Target 2)
K0130503	ABM	1.0 ug DNA	EUR 154

ASAH2 sgRNA CRISPR Lentivector (Human) (Target 3)
K0130504	ABM	1.0 ug DNA	EUR 154

Mouse ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)
ELI-34287m	Lifescience Market	96 Tests	EUR 865

Human ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)
ELI-34330h	Lifescience Market	96 Tests	EUR 824

Rat ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM)
ELI-48960r	Lifescience Market	96 Tests	EUR 886

Asah2 sgRNA CRISPR Lentivector (Rat) (Target 1)
K7087002	ABM	1.0 ug DNA	EUR 154

Asah2 sgRNA CRISPR Lentivector (Rat) (Target 2)
K7087003	ABM	1.0 ug DNA	EUR 154

Asah2 sgRNA CRISPR Lentivector (Rat) (Target 3)
K7087004	ABM	1.0 ug DNA	EUR 154

ASAH2 3'UTR Luciferase Stable Cell Line
TU001220	ABM	1.0 ml	EUR 1521

Asah2 3'UTR GFP Stable Cell Line
TU250951	ABM	1.0 ml	Ask for price

Asah2 3'UTR Luciferase Stable Cell Line
TU200951	ABM	1.0 ml	Ask for price

Asah2 3'UTR GFP Stable Cell Line
TU152183	ABM	1.0 ml	Ask for price

Asah2 3'UTR Luciferase Stable Cell Line
TU102183	ABM	1.0 ml	Ask for price

ASAH2 3'UTR GFP Stable Cell Line
TU051220	ABM	1.0 ml	EUR 1521

ASAH2 Protein Vector (Human) (pPB-C-His)
PV049013	ABM	500 ng	EUR 481

ASAH2 Protein Vector (Human) (pPB-N-His)
PV049014	ABM	500 ng	EUR 481

ASAH2 Protein Vector (Human) (pPM-C-HA)
PV049015	ABM	500 ng	EUR 481

ASAH2 Protein Vector (Human) (pPM-C-His)
PV049016	ABM	500 ng	EUR 329

ASAH2 Protein Vector (Human) (pPB-His-MBP)
PV324138	ABM	500 ng	EUR 481

ASAH2 Protein Vector (Human) (pPB-His-GST)
PV324139	ABM	500 ng	EUR 481

ASAH2 Protein Vector (Rat) (pPB-C-His)
PV254814	ABM	500 ng	EUR 1166

ASAH2 Protein Vector (Rat) (pPB-N-His)
PV254815	ABM	500 ng	EUR 1166

ASAH2 Protein Vector (Rat) (pPM-C-HA)
PV254816	ABM	500 ng	EUR 1166

ASAH2 Protein Vector (Rat) (pPM-C-His)
PV254817	ABM	500 ng	EUR 1166

ASAH2 Protein Vector (Mouse) (pPB-C-His)
PV156550	ABM	500 ng	EUR 1065

ASAH2 Protein Vector (Mouse) (pPB-N-His)
PV156551	ABM	500 ng	EUR 1065

ASAH2 Protein Vector (Mouse) (pPM-C-HA)
PV156552	ABM	500 ng	EUR 1065

ASAH2 Protein Vector (Mouse) (pPM-C-His)
PV156553	ABM	500 ng	EUR 1065

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit
20-abx156834	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) CLIA Kit
20-abx494694	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit
SEE689Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids.

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit
SEE689Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids.

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit
SEE689Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids.

Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit
SEE689Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids.

Subsequently, our device helped us to place the sunshine on new rules of KRAS activation throughout most cancers initiation. The usage of this device by the RAS group may contribute to discovering novel points of KRAS biology.