Traditional process scale-up using baculovirus is notoriously problematic, with yields dropping progressively during scale-up. SynBac is minimized baculoviral genomes created using modern synthetic biology techniques.
The SynBac project aims to transform fermenter-scale baculoviral production of drug targets, and manufacturing scale production of biopharmaceuticals by creating minimal baculoviral genomes displaying exceptional DNA stability. In addition to improved DNA stability, our minimized genomes form the starting point for applications that require large DNA cargo delivery to mammalian cells, primary cells, or organs.
SYNBAC is a clear blue antibacterial liquid hand soap with a neutral odor specially formulated for use in food handling applications. Directions for use: For one step hand-washing and sanitizing, wet hands and place approximately 5 ml in the palm of hand and lather thoroughly in a normal manner. Ensure contact under nails, back of hand, and wrists. Allow for at least one minute of contact time before rinsing your hands.
Baculovirus expression vector systems (BEVS) are widely used to produce heterologous proteins for a wide range of applications. Developed more than 30 years ago, BEVS has been constantly modified to improve product quality and ease of use. Plasmid reagents were tailored and engineered to facilitate the introduction of heterologous genes into baculoviral genomes. At the same time, detrimental modalities such as genes encoding proteases or apoptotic factors were removed to improve protein yield.
Advances in DNA synthesis and manipulation now enable the engineering of part or whole synthetic baculovirus genomes, opening up new avenues to redesign and tailor the system to specific applications. Here, we describe a simple protocol for designing and constructing baculovirus genomes comprising segments of synthetic DNA through the use of iterative Red/ET homologous recombination reactions.