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High Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat

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Technology of Excessive Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat α s1-Casein

The chemical, technological and allergy properties of goat’s milk are considerably affected by the extent of αs1-casein. Detection and quantification of αs1-casein requires high-specificity strategies to beat high-sequence similarity between this protein and others within the casein household. Unavailability of antibodies with excessive affinity and specificity in direction of goat αs1-casein hinders the event of immuno-based analytical strategies equivalent to enzyme-linked immunosorbent assay (ELISA) and biosensors.
  • Right here, we report the era of polyclonal antibodies (or immunoglobulins, IgGs) raised in direction of goat αs1-casein N- (Nter) and C-terminal (Cter) peptide sequences. The Nter and Cter peptides of goat αs1-casein had been immunized in rabbits for the era of antisera, which had been purified utilizing protein G affinity chromatography.
  • The binding affinity of the antisera and purified IgGs had been examined and in contrast utilizing oblique ELISA, the place peptide-BSA conjugates and goat αs1-casein had been used because the coating antigens. The Nter antiserum displayed increased titer than Cter antiserum, at 1/64,000 and 1/32,000 dilutions, respectively.
  • The purification step additional yielded 0.5 mg/mL of purified IgGs from Three mL of antisera. The purified Nter IgG confirmed a considerably (p < 0.05) increased binding affinity in direction of peptide-BSA and goat αs1-casein, with decrease Okayd worth at 5.063 × 10-3 μM in comparison with 9.046 × 10-3 μM for the Cter IgG.
  • A cross-reactivity check confirmed that there was no binding in neither Nter nor Cter IgGs in direction of protein extracts from the milk of cow, buffalo, horse and camel. Excessive-quality antibodies generated will permit additional improvement of immuno-based analytical strategies and future in vitro research to be carried out on goat αs1-casein.
Key phrases: IgG purification; anti-peptide polyclonal antibody; goat’s milk allergy; immunogenic peptides; αs1-casein.
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Fluorescein isothiocyanate (FITC) labelled anti-rabies virus ribonucleoprotein (RNP) antibodies can be utilized as immunoreagents in direct fluorescent antibody testing (dFAT) for rabies diagnoses. Whereas in-house merchandise are sometimes utilized by laboratories, most conjugates are business reagents. Business anti-RNP antibodies are solely accessible for analysis functions in Brazil, nevertheless, which contributes to the rising use of in-house produced antibodies.

Contemplating that conjugate high quality might affect the outcomes obtained throughout rabies prognosis, we sought to investigate the efficiency necessities of in-house produced polyclonal anti-RNP IgG-FITC for utility in dFAT. To that finish, their reproducibility, diagnostic sensitivity, and specificity had been evaluated. The titer of polyclonal anti-RNP IgG-FITC was initially decided and evaluated by dFAT, utilizing central nervous system (CNS) samples of various animal species (canines, cats, bovines, equines, bats, and non-human primates).

As our major consequence, the polyclonal anti-RNP IgG-FITC reached a titer of 1:30/1:40 in dFAT, with 100% of diagnostic sensitivity and specificity. When it comes to reproducibility, the antibodies, regardless the manufacturing batch, offered the identical performances. In conclusion, the in-house produced polyclonal anti-RNP IgG-FITC proved appropriate for rabies virus antigen detection by dFAT

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abx034845-400ul 400 ul
EUR 627.6

Netrin-1 (NTN1) Antibody
abx034845-80l 80 µl
EUR 343.2

Netrin-1 (NTN1) Antibody
20-abx338546
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Netrin-1 (NTN1) Antibody
20-abx329984
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Netrin-4 (NTN4) Antibody
abx432030-200ul 200 ul
EUR 343.2

Netrin-1 (NTN1) Antibody
20-abx327470
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Netrin 1 (Ntn1) Antibody
20-abx177738
  • EUR 493.20
  • EUR 159.60
  • EUR 1378.80
  • EUR 678.00
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Netrin 1 (Ntn1) Antibody
20-abx177739
  • EUR 1412.40
  • EUR 693.60
  • 1 mg
  • 200 ug

Netrin 1 (Ntn1) Antibody
20-abx177740
  • EUR 1496.40
  • EUR 710.40
  • 1 mg
  • 200 ug

Netrin 1 (Ntn1) Antibody
20-abx177741
  • EUR 526.80
  • EUR 159.60
  • EUR 1496.40
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Netrin 1 (NTN1) Antibody
abx217255-100ug 100 ug
EUR 526.8

Anti-Netrin G1 antibody
PAab05667 100 ug
EUR 426

Anti-Netrin 1 antibody
STJ70811 100 µg
EUR 430.8

Anti-Netrin G1 antibody
STJ71412 100 µg
EUR 312

Anti-Netrin-G1 antibody
STJ72988 100 µg
EUR 312

Anti-Netrin-1 antibody
STJ94407 200 µl
EUR 236.4
Description: Rabbit polyclonal to Netrin-1.

Anti-Netrin-1 antibody
STJ96887 200 µl
EUR 236.4
Description: Rabbit polyclonal to Netrin-1.

Netrin 1 Antibody / NTN1
RQ4809 100ul
EUR 419

Human Primary Antibody (IgG2) detection and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80170-G2 1 kit
EUR 781.2

Aflatoxin G2
A118-0.5MG 0.5 mg
EUR 188.4

Aflatoxin G2
A118-2.5MG 2.5 mg
EUR 567.6

g2-MSH
H-4400.0001 1.0mg
EUR 211.2
Description: Sum Formula: C74H99N21O16S; CAS# [72711-43-4]

g2-MSH
H-4400.0005 5.0mg
EUR 733.2
Description: Sum Formula: C74H99N21O16S; CAS# [72711-43-4]

Aflatoxin G2
TBW00771 1ml
EUR 375.6

Licoricesaponin G2
TBW01421 5mg Ask for price

Euglobal G2
TBZ1300 unit Ask for price

CD68(CD68/G2) Antibody
BNCA0919-250 250uL
EUR 459.6
Description: Primary antibody against CD68(CD68/G2), APC conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNCAP0919-100 100uL
EUR 238.8
Description: Primary antibody against CD68(CD68/G2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNCAP0919-500 500uL
EUR 652.8
Description: Primary antibody against CD68(CD68/G2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

EF-G2 Polyclonal Antibody
ABP56753-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of EF-G2 from Human. This EF-G2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human EF-G2 at AA range: 410-490

EF-G2 Polyclonal Antibody
ABP56753-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of EF-G2 from Human. This EF-G2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human EF-G2 at AA range: 410-490

EF-G2 Polyclonal Antibody
ABP56753-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of EF-G2 from Human. This EF-G2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human EF-G2 at AA range: 410-490

CD68(CD68/G2) Antibody
BNC610919-100 100uL
EUR 238.8
Description: Primary antibody against CD68(CD68/G2), CF660R conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC610919-500 500uL
EUR 652.8
Description: Primary antibody against CD68(CD68/G2), CF660R conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNUM0919-50 50uL
EUR 474
Description: Primary antibody against CD68(CD68/G2), 1mg/mL

CD68(CD68/G2) Antibody
BNCR0919-250 250uL
EUR 459.6
Description: Primary antibody against CD68(CD68/G2), RPE conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNUB0919-100 100uL
EUR 250.8
Description: Primary antibody against CD68(CD68/G2), Concentration: 0.2mg/mL

CD68(CD68/G2) Antibody
BNUB0919-500 500uL
EUR 549.6
Description: Primary antibody against CD68(CD68/G2), Concentration: 0.2mg/mL

CD68(CD68/G2) Antibody
BNC430919-100 100uL
EUR 238.8
Description: Primary antibody against CD68(CD68/G2), CF543 conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC430919-500 500uL
EUR 652.8
Description: Primary antibody against CD68(CD68/G2), CF543 conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC400919-100 100uL
EUR 238.8
Description: Primary antibody against CD68(CD68/G2), CF640R conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC400919-500 500uL
EUR 652.8
Description: Primary antibody against CD68(CD68/G2), CF640R conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC810919-100 100uL
EUR 238.8
Description: Primary antibody against CD68(CD68/G2), CF680R conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC810919-500 500uL
EUR 652.8
Description: Primary antibody against CD68(CD68/G2), CF680R conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC880919-100 100uL
EUR 238.8
Description: Primary antibody against CD68(CD68/G2), CF488A conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC880919-500 500uL
EUR 652.8
Description: Primary antibody against CD68(CD68/G2), CF488A conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC800919-100 100uL
EUR 238.8
Description: Primary antibody against CD68(CD68/G2), CF680 conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNC800919-500 500uL
EUR 652.8
Description: Primary antibody against CD68(CD68/G2), CF680 conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNCH0919-100 100uL
EUR 238.8
Description: Primary antibody against CD68(CD68/G2), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNCH0919-500 500uL
EUR 652.8
Description: Primary antibody against CD68(CD68/G2), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD68(CD68/G2) Antibody
BNCP0919-250 250uL
EUR 459.6
Description: Primary antibody against CD68(CD68/G2), PerCP conjugate, Concentration: 0.1mg/mL
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SynBac

Traditional process scale-up using baculovirus is notoriously problematic, with yields dropping progressively during scale-up. SynBac is minimized baculoviral genomes created using modern synthetic biology techniques. The SynBac project aims to transform fermenter-scale baculoviral production of drug targets, and manufacturing scale

Read More about SynBac
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Posted inAntibodies Assay Kits Biology Cells cDNA Clia Kits Culture Cells DNA Elisa Kits Enzymes Equipments Exosomes Isotypes Medium & Serums NATtrol Particles PCR Pcr Kits Peptides Recombinant Proteins Ria Kits RNA Test Kits Vector & Virus Western Blot

Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor

An immunobiosensor assay was developed for multi-residue screening in bovine milk of the mother or father amphenicols, thiamphenicol and florfenicol, together with the metabolite florfenicol amine. A polyclonal antibody raised in a rabbit after immunisation with a florfenicol amine-protein conjugate was employed within the

Read More about Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor
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